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Paper

Interferon-stimulated and metallothionein-expressing macrophages are associated with acute and chronic allograft dysfunction after lung transplantation

Evidence level: single-cell RNA sequencing study; abstract-only

Single-cell sequencing of lavage cells found two macrophage subsets, interferon-stimulated and metallothionein-expressing, linked to acute lung allograft dysfunction and also seen in explanted CLAD lungs.

As of . Primary source: Publisher record via DOI.

Summary

The study applied single-cell RNA sequencing to bronchoalveolar lavage cells from stable recipients and recipients with acute lung allograft dysfunction (ALAD), and to cells from explanted CLAD lung tissue. It identified two alveolar macrophage subsets found uniquely in ALAD, annotated as interferon-stimulated gene (ISG) and metallothionein-mediated inflammatory (MT) macrophages. In laboratory tests of an independent set of macrophages, ALAD macrophages showed higher expression of CXCL10 and secreted more pro-inflammatory cytokines than macrophages from stable patients. Analysis of cells from four explanted CLAD lungs showed similar macrophage populations. Donor and recipient cells both contributed to all macrophage subsets early after transplant, with loss of donor-derived cells over time. The authors suggest these subsets may be therapeutic targets. The numbers of patients in the lavage groups are not stated in the abstract.

Details

doi
10.1016/j.healun.2022.05.005
pmid
35691795
authors
Moshkelgosha S, Duong A, Wilson G, Andrews T, Berra G, Renaud-Picard B, Liu M, Keshavjee S, MacParland S, Yeung J, Martinu T, Juvet S
journal
The Journal of Heart and Lung Transplantation
year
2022
volume
41
issue
11
pages
1556-1569

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Information resource only. Not medical advice. Not a substitute for the care of the patient's transplant team.