GEO GSE274199: single-cell analysis of acute cellular rejection and fibrogenic shift in lung allografts

Single-cell RNA and ATAC sequencing of lung allograft biopsies from 8 paediatric and young adult patients with and without acute cellular rejection.

As of 2026-10-10. Primary source: GEO series GSE274199.

Summary

The GEO series record states: single-cell RNA sequencing of freshly collected lung tissue from 8 paediatric and young adult patients with acute cellular rejection (ACR), resolved ACR and surveillance biopsies without ACR, with validation by microarray, immunofluorescence and single-cell ATAC-seq. The record reports persistent TGF-beta and PI3K/AKT/mTOR pathway activation after histological resolution. Status public on 27 February 2026; contact institute Cincinnati Children's Hospital Medical Center. The record states that raw human files are available through controlled access in dbGaP rather than in GEO.

Details

accession: GSE274199
repository: GEO
assay: Expression profiling by high throughput sequencing; genome binding profiling (scATAC-seq); other
n: 8 patients (69 samples listed in GEO)
organism: Homo sapiens
access: Raw human sequence files are under controlled access in dbGaP, not open in GEO (per the series record).

Sources and links

GEO series GSE274199 (primary)

Links from this record

profiles: Acute cellular rejection

Linked from (derived)

evidence: Human tissue studies are small and raw human sequence data are often not open

Known gaps in this record

licence or reuse terms
link to the associated paper record (the GEO record lists a PubMed identifier that was not opened)
data access route beyond the statement in the series record

Information resource only. Not medical advice. Not a substitute for the care of the patient's transplant team.